INCREDB ID : 310
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Rajeshwar N. Sharan, Ph.D.
Professor 
 
North Eastern Hill University
Department of Biochemistry, Radiation and Molecular Biology Unit
Umshing,
Shillong - 793 022   
Meghalaya   
 
E-mail: rnsharan@nehu.ac.in
Phone: +91-364-2722121, +91-364-2550108
 
URL: http://www.nehu.ac.in/Schools/Life%20Sciences/Biochemistry/faculty.pdf
 
Positions Held:
Position Affiliation Time Period
Professor North Eastern Hill University, Shillong, India 2000-Present
Visiting Professor Osaka University, Osaka, Japan Present
Visiting Professor Forschungszentrum Juelich, Germany Present
Head of Department North Eastern Hill University, Shillong, India 1996-1999
Reader North Eastern Hill University, Shillong, India 1992
Post Doctoral Fellow Research Center Juelich, Germany 1987-1988
Lecturer North Eastern Hill University, Shillong, India 1984
 
Area of Specialization:
Biochemistry
Research Summary:
Understanding the role of poly-ADP-ribosylation of chromosomal proteins and its suitability for a population detection/screening tool for cancer
Betel nut carcinogenesis
HMG proteins in regulation of gene expression during induced carcinogenesis by betel nut, chemical carcinogens and radiation of different qualities
Chemical modification of radiation and chemical carcinogenesis at molecular levels and drug carrier system and development
 
Publications:
1. Choudhury, Y. and Sharan, R. N. Altered BRCA1 and BRCA2 responses and mutation of BRCA1 gene in mice exposed chronically and transgenerationally to aqueous extract of betel nut (AEBN). Environmental Toxicology and Pharmacology. 2011. 31, 57-69.
2. Lakadong, R. O., Kataki, A. C. and Sharan, R. N.  ADP-ribose polymer--a novel and general biomarker of human cancers of head & neck, breast, and cervix. Molecular Cancer. 2010.  9, 286. [PubMed]
3. Turtoi, A., Sharan, R. N., Srivastava, A. and Schneeweiss, F. H.  Proteomic and genomic modulations induced by γ-irradiation of human blood lymphocytes. International Journal of Radiation Biology. 2010.  86, 888-904. [PubMed]
4. Choudhury, Y. and Sharan, R. N.  Ultrastructural alterations in liver of mice exposed chronically and transgenerationally to aqueous extract of betel nut: Implications in betel nut-induced carcinogenesis. Microscopy Research and Technique. 2010.  73, 530-539. [PubMed]
5. Choudhury, Y. and Sharan, R. N. Altered p53 response and enhanced transgenerational transmission of carcinogenic risk upon exposure of mice to betel nut. Environmental Toxicology and Pharmacology. 2009. 27, 127-138.
6. Bhattacharjee, C. and Sharan, R. N. Aqueous extract of betel nut-induced adducts on pMTa4 DNA acquires stability in the presence of Na+ and K+ ions. Molecular Medicine Reports. 2008. 1, 435-441.
7. Kma, L. and Sharan, R. N. Chronic exposure of mice to aqueous extract of betel nuts inhibits poly-ADP-ribosylation of total cellular proteins as well as histone H1 protein. Canadian Journal of Pure and Applied Sciences. 2008. 2, 123-128.
8. Sharan, R. N., Ryo, H. and Nomura, T.  Critical role of RecA and RecF proteins in strand break rejoining and maintenance of fidelity of rejoining following gamma-radiation-induced damage to pMTa4 DNA in E. coli. International Journal of Radiation Biology. 2007.  83, 89-97. [PubMed]
9. Turtoi, A., Srivastava, A., Sharan, R. N., Oskamp, D., Hille, R. and Schneeweiss. Early response of lymphocyte proteins after γ-radiation. Journal of Radioanalytical and Nuclear Chemistry. 2007. 274, 435-439.
10. Jaylata, D. and Sharan, R. N.  Progressive reduction in poly-ADP-ribosylation of histone proteins during Dalton's lymphoma induced ascites tumorigenesis in mice. Cancer Letters. 2006.  238, 135-141. [PubMed]
11. Kma, L. and Sharan, R. N.  In vivo exposure of swiss albino mice to chronic low dose of dimethylnitrosamine (DMN) lowers poly-ADP-ribosylation (PAR) of bone marrow cell and blood lymphocyte proteins. Molecular and Cellular Biochemistry. 2006.  288, 143-149. [PubMed]
12. Bhattacharjee, C. and Sharan, R. N.  UV-C radiation induced conformational relaxation of pMTa4 DNA in Escherichia coli may be the cause of single strand breaks. International Journal of Radiation Biology. 2005.  81, 919-927. [PubMed]
13. Sharan, R. N., Devi, B. J., Humtsoe, J. O., Saikia, J. R. and Kma, L.  Detection and quantification of poly-ADP-ribosylated cellular proteins of spleen and liver tissues of mice in vivo by slot and Western blot immunoprobing using polyclonal antibody against mouse ADP-ribose polymer. Molecular and Cellular Biochemistry. 2005.  278, 213-221. [PubMed]
14. Meriyani, O. and Sharan, R. N.  Differential DNA strand breaking abilities of *OH and ROS generating radiomimetic chemicals and gamma-rays: study of plasmid DNA, pMTa4, in vitro. Free Radical Research. 2005.  39, 499-505. [PubMed]
15. Alam, A., Rapthap, C., Singha, L. I., Sharan, R. N. and Singh, V.  Radiomodulatory effect of liposome encapsulated AK-2123 on tumor in mice exposed to hepatocarcinogen. Molecular and Cellular Biochemistry. 2005.  271, 139-150. [PubMed]
16. Devi, B. J., Schneeweiss, F. H. and Sharan, R. N.  Negative correlation between poly-ADP-ribosylation of spleen cell histone proteins and initial duration of dimethylnitrosamine exposure to mice in vivo measured by Western blot immunoprobe assay: a possible biomarker for cancer detection. Cancer Detection and Prevention. 2005.  29, 66-71. [PubMed]
17. Sharan R. N.  Preparedness to respond to possible acts of nuclear terrorism: some strategies and recommendations. International Journal of Radiation Biology. 2003.  79, 217-219. [PubMed]
18. Humtsoe, J. O., Schneeweiss, F. H. A., Srivastava, A., Sarma and Sharan, R. N. Biological effects induced by swift heavy ions of lithium on aqueous solution of plasmid pMTa4. Radiation Effects and Defects in Solids. 2003. 158, 603-607.
19. Pariat, T. and Sharan, R. N. Role of mouse spleen cell HMG proteins and their poly-ADP-ribosylation in betelnut induced carcinogenesis. Indian Journal of Biophysics and Biochemistry. 2002. 39, 130-132.
20. Sharan, R. N. Nuclear terrorism perception: some strategies and recommendations to handle a disaster scenario. Current Science. 2002. 85, 977-978.
21. Schneeweiss, F. H., Mukherjee, A. S., Sharan, R. N., Feinendegen, L. E. and Muller-Gartner, H. W.  Response of high mobility group proteins of human kidney T1 and murine L 929 cell lines to heat shock. Life Sciences. 2000.  66, 193-200. [PubMed]
22. Schneeweiss, F. H. A., Mukherjee, A. S., Sharan, R. N. and Feinedegen, L. E. Heat shock induced chromatin conformational changes: HMG depletion and Dnase I sensitivity. Life Sciences. 2000. 63, 193-200.
23. Schneeweiss, F. H., Sharan, R. N. and Brown, I.  211At-alpha-dose dependence of poly-ADP-ribosylation of human glioblastoma cells in vitro. Suitability in cancer therapy? Strahlentherapie Und Onkologie. 1999.  175, 458-461. [PubMed]
24. Saikia, J. R., Schneeweiss, F. H. and Sharan, R. N.  Arecoline-induced changes of poly-ADP-ribosylation of cellular proteins and its influence on chromatin organization. Cancer Letters. 1999.  139, 59-65. [PubMed]
25. Schneeweiss, F. H. A. and Sharan, R. N. Poly-ADP-ribosylation of proteins responds to cellular perturbations. Recent Aspects of Cellular and Applied Radiobiology. 1999. 62-71.
26. Schneeweiss, F. H. A., Sharan, R. N., Hautzel, H. and Müller-Gärtner, H. W. Effect of γ-radiation on the ratio of [18F]2-fluoro-2-deoxy-D-glucose to glucose utilization in human glioblastoma cells in vitro. Journal of Biosciences. 1999. 24, 49-52.
27. Sharan, R. N., Schneeweiss, F. H., Saikia, J. R. and Feinendegen, L. E.  Poly-ADP-ribosylation of histone proteins of human kidney T1-cells in vitro following gamma-irradiation. Indian Journal of Biochemistry & Biophysics. 1998.  35, 97-102. [PubMed]
28. Saikia, J. R., Schneeweiss, F. H. A. and Sharan, R. N. Effects of chronic low-dose arecoline administration on the macromolecular components of bone marrow and spleen cells of mice. The Cancer Journal. 1998. 11, 94-98.
29. Pariat, T. and Sharan, R. N. Qualitative changes in mice liver HMG proteins after low dose chronic administration of aqueous extract of betel nut and diethylnitrosamine. Hepatoogy Research. 1998. 12, 177-185.
30. Bohm, L., Schneeweiss, F. A., Sharan, R. N. and Feinendegen, L. E.  Influence of histone acetylation on the modification of cytoplasmic and nuclear proteins by ADP-ribosylation in response to free radicals. Biochimica et Biophysica Acta. 1997.  1334, 149-154. [PubMed]
31. Sharan, R. N., Schneeweiss, F. H. and Feinendegen, L. E.  Neutrons affect ADP-ribosylation of proteins in human kidney T1-cells in vitro. Indian Journal of Biochemistry & Biophysics. 1996.  33, 281-284. [PubMed]
32. Sharan, R. N. Association of betel nut with carcinogenesis.The Cancer Journal. 1996. 9, 13-19.
33. Pariat, T. and Sharan, R. N.  Low dose exposure of diethylnitrosamine affects mice liver thymidine kinase. Life Sciences. 1995.  57, 2431-2437. [PubMed]
34. Schneeweiss, F. H., Sharan, R. N. and Feinendegen, L. E.  Change of ADP-ribosylation in human kidney T1-cells by various external stimuli. Indian Journal of Biochemistry & Biophysics. 1995.  32, 119-124. [PubMed]
35. Sharan, R. N., Alam, A., Chakraborty, S., Saikia, J. R. and Srivastava, P. N.  2-Mercaptopropionylglycine affords enhanced radioprotection after a liposome encapsulation. Journal of Radiation Research. 1995.  36, 31-37. [PubMed]
36. Schneeweiss, F. H. A., Sharan, R. N., Hautzel, H., Rub, T.H. and Mullergartner, H. W. Effect of Ionising radiation on the conversion factor between deoxyglucose and glucose (Lumped Constant) in human glioblastoma cells in vitro. The Journal of Nuclear Medicine. 1995. 36, 202.
37. Schneeweiss, F. H. A., Xia, F., Sharan, R. N. and Feinendegen, L. E. A strong static magnetic field inhibits the poly-ADP-ribosylation of proteins in human kidney T1-cells. Bioelectrochemistry and Bioenergetics. 1993. 30, 111-117.
38. Sharan, R. N. and Wary, K. K.  Study of unscheduled DNA synthesis following exposure of human cells to arecoline and extracts of betel nut in vitro. Mutation Research. 1992.  278, 271-276. [PubMed]
39. Sharan, R. N., Alam, A., Saikia, J. R., Chaudhury, S. and Srivastava, P. N. Liposome mediated delivery of 2-mercaptopropionyl glycine:entrapment of MPG in liposome. Radiosensitization Newsletter. 1992. 11, 16-17.
40. Wary, K. K. and Sharan, R. N.  Cytotoxic and cytostatic effects of arecoline and sodium nitrite on human cells in vitro. International Journal of Cancer. Journal International Du Cancer. 1991.  47, 396-400. [PubMed]
41. Sharan, R. N. MPG concentration dependent sensitisation of gamma induced DNA strand breaks in human lymphocytes in vitro. Radiosensitization Newsletter (Japan). 1990. 9, 8-9.
42. Wary, K. K. and Sharan, R. N.  Effect of the radioprotector 2-mercaptopropionylglycine (MPG) on the radiation inactivation of catalase in vitro. Journal of Radiation Research. 1988.  29, 104-109. [PubMed]
43. Wary, K. K. and Sharan, R. N.  Aqueous extract of betel-nut of north-east India induces DNA-strand breaks and enhances rate of cell proliferation in vitro. Effects of betel-nut extract in vitro. Journal of Cancer Research and Clinical Oncology. 1988.  114, 579-582. [PubMed]
44. Wary, K. K., Lalthanpuia and Sharan, R. N. 2-mercaptopropionyl glycine (MPG) induced protection of DNA damage in Gamma-irradiated human lymphocytes. Radiosensitization Newsletter (Japan). 1988. 8, 4-6.
45. Kapoor, G., Sharan, R. N. and Srivastava, P. N.  Histopathological changes in the ovary following acute and chronic low-level tritium exposure to mice in vivo. International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine. 1985.  47, 197-203. [PubMed]
46. Sharan, R. N. and Srivastava, P. N.  Effects of tritiated water ingestion on mice: III. Hexokinase isozymes in brain, liver and spleen up to five generations. International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine. 1984.  46, 83-93. [PubMed]
47. Sharan, R. N. and Srivastava, P. N.  Effects of tritiated water ingestion on mice: I. Hexokinase activity changes in brain and liver. Journal of Radiation Research. 1980.  21, 231-238. [PubMed]

 
Book Chapters:
Sharan, R. N. Poly-ADP-ribosylation in cancer. In: Cancer Epigenetics. Tollefsbol, T., Editor. CRC Press, USA. 2009. Chapter 15. pp 265-279.
Chakraborty, S., Rapthap, C., Alam, A., Srivastava, P. N. and Sharan, R. N. Liposome as a carrier for delivery of radiomodulatory drugs and Its advantages in chemoradiotherapy. In: Radiation Radiomodifiers and Human Health. Devi, P. U., Bisht, K. S. and Rao, B. S. S., Eds. NISCOM, New Delhi. 2005. pp 111-123.
Srivastava, P. N. and Sharan, R. N. Need and importance of further research in the field of radiation. In: Life sciences and radiation. Kiefer, J., Editor. Springer-Verlag, Germany. 2004. pp 259.
Humtsoe, J. O. and Sharan, R. N. Molecular radiobiology: Plasmid pMTa4 as a Tool for studying effects of g-radiation in vitro and in vivo. In: Radiobiology and Bio-Medical Research. Mishra, K. P., Editor. Narosa Publishing House, India. 2004. pp 51-61.
Schneeweiss, F. H. A., Zerhusen, S. and Sharan, R. N. Effects of angur-elect-emitting radionuclides on human cells. In: Radiobiology and Bio-Medical Research. Mishra, K. P., Editor. Narosa Publishing House, India. 2004. pp 66-71.
Schneeweiss, F. H. A., and Sharan, R. N. Study of effects of radiation on poly-ADPribosylation: what have we learnt? In: Recent Aspects of Fundamental and Applied Radiobiology. Schneeweiss, F. H. A. and Sharan, R. N., Eds. Forschungszentrum Juelich GmbH, Germany. 1999. International Cooperation Bilateral Seminars series. Volume 30. pp 62-72.
Alam, A., Chakraborty, S., Rapthap, C., Srivastava, P. N. and Sharan, R. N. Enhancement of radiomodulatory effect through liposome encapsulated radiomodifiers on cancer bearing mice. In: Recent Aspects of Fundamental and Applied Radiobiology. Schneeweiss, F. H. A. and Sharan, R. N., Eds. Forschungszentrum Juelich GmbH, Germany. 1999. International Cooperation Bilateral Seminars series. Volume 30. pp 83-88.
Saikia, J. R., Schneeweiss, F. H. A. and Sharan, R. N. Chronic low dose arecoline exposure affects DNA, RNA and proteins contents and cellular Poly-ADP-ribosylation in mice in vivo. In: Recent Aspects of Fundamental and Applied Radiobiology. Schneeweiss, F. H. A. and Sharan, R. N., Eds. Forschungszentrum Juelich GmbH, Germany. 1999. International Cooperation Bilateral Seminars series. Volume 30. pp 149-152.
Pariat, T., Balachandran, B. and Sharan, R. N. Effects of carcinagen exposure on Poly-ADP-ribosylation of HMG proteins and on chromatin organization. In: Recent Aspects of Fundamental and Applied Radiobiology. Schneeweiss, F. H. A. and Sharan, R. N., Eds. Forschungszentrum Juelich GmbH, Germany. 1999. International Cooperation Bilateral Seminars series. Volume 30. pp 158-161.
Sharan, R. N., Editor. Trends in Radiation and Cancer Biology. Forschungszentrum Juelich GmbH, Germany. 1998. International Cooperation Bilateral Seminars series. Volume 29.
Sinha, T. K. and Sharan, R. N. A model for DNA and DNA repair. In: Trends in Radiation and Cancer Biology. Sharan, R. N., Editor. Forschungszentrum Juelich GmbH, Germany. 1998. International Cooperation Bilateral Seminars series. Volume 29. pp 128-134.
Pariat, T. and Sharan, R. N. Betel nut and diethylnitrosamine induced conformational changes in the liver HMG proteins in vivo. In: Trends in Radiation and Cancer Biology. Sharan, R. N., Editor. Forschungszentrum Juelich GmbH, Germany. 1998. International Cooperation Bilateral Seminars series. Volume 29. pp 162-165.
Rapthap, C., Imliwati, L. K., Sharan, R. N. and Alam, A. Tumor-associated antigen expression in mice exposed to diethylnitrosamine. In: Trends in Radiation and Cancer Biology. Sharan, R. N., Editor. Forschungszentrum Juelich GmbH, Germany. 1998. International Cooperation Bilateral Seminars series. Volume 29. pp 177-180.
Sharan, R. N., Devi, B. J., Humtsoe, J. O. and Schneewiess, F. H. A. Immunodetection of cellular Poly-ADP-ribosylation. In: Trends in Radiation and Cancer Biology. Sharan, R. N., Editor. Forschungszentrum Juelich GmbH, Germany. 1998. International Cooperation Bilateral Seminars series. Volume 29. pp 240-243.
Sharan, R. N. Biochemical investigation of carcinogenic potency of betel nut (Kwai) of North-East India. In: Oral Oncology. MacMillan India Ltd. 1994. Volume III. pp 190-193.
Schneeweiss, F. H. A., Xia, F., Sharan, R. N. and Feinendegen. Influence of static magnetic field on the poly-ADP-ribosytation of plasma and nuclear proteins of human kidney T1-cells. In: Progress in Radiation Protection. Verlag TUV Publishers, Rheinland. 1991. Volume I. pp 434-438.
Sharan, R. N. and Srivastava, P. N. Effect of tritiated water ingestion on mice: III. Isozymes of hexokinase. In: Radiation Research, volume E - Dosimetry, Radionuclides and Technology. Broerse, J. J., Barendsen, G. W., Kal, H. B. and Kogel van der, A. J., Eds. Martinus Nijhoff Publishers, The Netherlands. 1983. C2-13.
Srivastava, P. N., Kapoor, G. and Sharan, R. N. Effect of acute and chronic low-level tritium exposure on the ovary of mice in vivo. In: Radiation Research, volume E - Dosimetry, Radionuclides and Technology. Broerse, J. J., Barendsen, G. W., Kal, H. B. and Kogel van der, A. J., Eds. Martinus Nijhoff Publishers, The Netherlands. 1983. E5-14.
Srivastava, P. N., Sharan, R. N. and Pozzi, L. Effects of tritiated water ingestion on mice: II. Damage at cellular vis-a-vis subcellular level monitored upto four generations. In: Advances in Nuclear Medicine and Biology. Pergamon Press, Oxford. 1983. 4, 2989-2992.
 
Grant Support:
Project Title Role Funding Agency Sanction Number Sanction Date
Development of appropriate models of DNA during radiation damage and repair 2000-2001
Molecular biology of radiation damage and repair Department of Science & Technology, Government of India 1998-2002
Poly-ADP ribosylation in cellular transformation Department of Science & Technology, Government of India 1997-2001
High Mobility Group (HMG) proteins in carcinogenesis Council of Scientific and Industrial Research, Government of India 1991-1995
Response protein University Grants Commission, India 1986-1990
Betel nut carcinogenesis 1986-1988

 
 
My CV:   Download
 
Education 
::
M.Sc. Jawaharlal Nehru University, New Delhi, India
::
Ph.D. Jawaharlal Nehru University, New Delhi, India
Member
::
2002-2004, President, Indian Society for Radiation Biology
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1990-1993, Member, National Youth Council, Govt. of India
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Associate Editor - Journal of Radiation Research
  Last modified on : Mar 4, 2011
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