Subrata Sinha, M.D., Ph.D.
National Brain Research Centre
Manesar
Gurgaon - 122 050
Haryana
E-mail: subrata.sinha@nbrc.ac.in, director@nbrc.ac.in
Positions Held:
Position Affiliation Time Period
Director
All India Institue of Medical Sciences, New Delhi, India
Professor and Head
All India Institue of Medical Sciences, New Delhi, India
Area of Specialization:
Molecular Oncology
Immunology
Research Summary:
Generation of potentially immunotherapeutic antibodies against target antigens from phage display libraries
Molecular biology of glial and tumourigenesis
Publications:
1.
Shahi, M. H., Afzal, M., Sinha, S. , Eberhart, C. G., Rey, J. A., Fan, X. and Castresana, J. S. Regulation of sonic hedgehog-GLI1 downstream target genes PTCH1, Cyclin D2, Plakoglobin, PAX6 and NKX2.2 and their epigenetic status in medulloblastoma and astrocytoma. BMC Cancer. 2010. 10, 614. [PubMed]
2.
Shahi, M. H., Afzal, M., Sinha, S. , Eberhart, C. G., Rey, J. A., Fan, X. and Castresana, J. S. Human hedgehog interacting protein expression and promoter methylation in medulloblastoma cell lines and primary tumor samples. Journal of Neuro-oncology. 2010. [PubMed]
3.
Shahi, M. H., Schiapparelli, P., Afzal, M., Sinha, S. , Rey, J. A. and Castresana, J. S. Expression and epigenetic modulation of sonic hedgehog-GLI1 pathway genes in neuroblastoma cell lines and tumors. Tumour Biology . 2011. 32, 113-127. [PubMed]
4.
Tiwari, A., Sankhyan, A., Khanna, N. and Sinha, S. Enhanced periplasmic expression of high affinity humanized scFv against Hepatitis B surface antigen by codon optimization. Protein Expression and Purification. 2010. 74, 272-279. [PubMed]
5.
Palanichamy, J. K., Mehndiratta, M., Bhagat, M., Ramalingam, P., Das, B., Das, P., Sinha, S. and Chattopadhyay, P. Silencing of integrated human papillomavirus-16 oncogenes by small interfering RNA-mediated heterochromatization. Molecular Cancer Therapeutics. 2010. 9, 2114-2122. [PubMed]
6.
Ahmad, Y., Bhatia, M. S., Mediratta, P. K., Sharma, K. K., Negi, H., Chosdol, K. and Sinha, S. Association between the ionotropic glutamate receptor kainate3 (GRIK3) Ser310Ala polymorphism and schizophrenia in the Indian population. The World Journal of Biological Psychiatry : the Official Journal of the World Federation of Societies of Biological Psychiatry. 2009. 10, 330-333. [PubMed]
7.
Goswami, P., Saini, D. and Sinha, S. Phage displayed scFv: pIII scaffold may fine tune binding specificity. Hybridoma. 2009. 28, 327-331. [PubMed]
8.
Puri, S., Puri, S., Mahapatra, A. K., Hussain, E., Sarkar, C., Sinha, S. and Joshi, B. H. A review of studies on targeting interleukin 4 receptor for central nervous system malignancy. Current Molecular Medicine. 2009. 9, 732-739. [PubMed]
9.
Pal, A., Srivastava, T., Sharma, M. K., Mehndiratta, M., Das, P., Sinha, S. and Chattopadhyay, P. Aberrant methylation and associated transcriptional mobilization of Alu elements contributes to genomic instability in hypoxia. Journal of Cellular and Molecular Medicine. 2010. 14, 2646-2654. [PubMed]
10.
Tiwari, A., Khanna, N., Acharya, S. K. and Sinha, S. Humanization of high affinity anti-HBs antibody by using human consensus sequence and modification of selected minimal positional template and packing residues. Vaccine. 2009. 27, 2356-2366. [PubMed]
11.
Tiwari, A., Dutta, D., Khanna, N., Acharya, S. K. and Sinha, S. Generation and characterization of high affinity humanized fab against hepatitis B surface antigen. Molecular Biotechnology. 2009. 43, 29-40. [PubMed]
12.
Mehndiratta, M., Palanichamy, J. K., Ramalingam, P., Pal, A., Das, P., Sinha, S. and Chattopadhyay, P. Fluorescence acquisition during hybridization phase in quantitative real-time PCR improves specificity and signal-to-noise ratio. BioTechniques. 2008. 45, 625-631. [PubMed]
13.
Ahmad, Y., Bhatia, M. S., Mediratta, P. K., Sharma, K. K., Negi, H., Chosdol, K. and Sinha, S. Association between the ionotropic glutamate receptor kainate3 (GRIK3) Ser310Ala polymorphism and schizophrenia in the Indian population. The World Journal of Biological Psychiatry. 2009. , 1-4. [PubMed]
14.
Chosdol, K., Misra, A., Puri, S., Srivastava, T., Chattopadhyay, P., Sarkar, C., Mahapatra, A. K. and Sinha, S. Frequent loss of heterozygosity and altered expression of the candidate tumor suppressor gene 'FAT' in human astrocytic tumors. BMC Cancer. 2009. 9, 5. [PubMed]
15.
Misra, A., Chattopadhyay, P., Chosdol, K., Sarkar, C., Mahapatra, A. K. and Sinha, S. Clonal mutations in primary human glial tumors: evidence in support of the mutator hypothesis. BMC Cancer. 2007. 7, 190. [PubMed]
16.
Mondal, A. M., Chinnadurai, S., Datta, K., Chauhan, S. S., Sinha, S. and Chattopadhyay, P. Identification and functional characterization of a novel unspliced transcript variant of HIC-1 in human cancer cells exposed to adverse growth conditions. Cancer Research. 2006. 66, 10466-10477. [PubMed]
17.
Srivastava, T., Chosdol, K., Chattopadhayay, P., Sarkar, C., Mahapatra, A. K. and Sinha, S. Frequent loss of heterozygosity encompassing the hMLH1 locus in low grade astrocytic tumors. Journal of Neuro-oncology. 2007. 81, 249-255. [PubMed]
18.
Mishra, S. and Sinha, S. Prediction and molecular modeling of T-cell epitopes derived from placental alkaline phosphatase for use in cancer immunotherapy. Journal of Biomolecular Structure & Dynamics. 2006. 24, 109-121. [PubMed]
19.
Bose, B., Khanna, N., Acharya, S. K. and Sinha, S. Generation and characterization of a single-gene mouse-human chimeric antibody against hepatitis B surface antigen. Journal of Gastroenterology and Hepatology. 2006. 21, 1439-1447. [PubMed]
20.
Bose, B., Khanna, N., Acharya, S. K. and Sinha, S. High affinity mouse-human chimeric Fab against hepatitis B surface antigen. World Journal of Gastroenterology. 2005. 11, 7569-7578. [PubMed]
21.
Saini, D., Kala, M., Jain, V. and Sinha, S. Targeting the active site of the placental isozyme of alkaline phosphatase by phage-displayed scFv antibodies selected by a specific uncompetitive inhibitor. BMC Biotechnology. 2005. 5, 33. [PubMed]
22.
Bose, B., Khanna, N., Acharya, S. K. and Sinha, S . Biotechnology and Applied Biochemistry. 2006. 43, 93-101. [PubMed]
23.
Bose, B. and Sinha, S. Problems in using statistical analysis of replacement and silent mutations in antibody genes for determining antigen-driven affinity selection. Immunology. 2005. 116, 172-183. [PubMed]
24.
Sarkar, C., Karak, A. K., Nath, N., Sharma, M. C., Mahapatra, A. K., Chattopadhyay, P. and Sinha, S. Apoptosis and proliferation: correlation with p53 in astrocytic tumours. Journal of Neuro-oncology. 2005. 73, 93-100. [PubMed]
25.
Srivastava, T., Seth, A., Datta, K., Chosdol, K., Chattopadhyay, P. and Sinha, S. Inter-alu PCR detects high frequency of genetic alterations in glioma cells exposed to sub-lethal cisplatin. International Journal of Cancer. 2005. 117, 683-689. [PubMed]
26.
Puri, S., Joshi, B. H., Sarkar, C., Mahapatra, A. K., Hussain, E. and Sinha, S. Expression and structure of interleukin 4 receptors in primary meningeal tumors. Cancer. 2005. 103, 2132-2142. [PubMed]
27.
Sarkar, C., Sinha, S., Sharma, M. C., Kumar, R. and Mehta, V. S. Supratentorial glioblastoma in adults: identification of subsets and their clinical correlation. Brain Tumor Pathology. 2004. 21, 7-12. [PubMed]
28.
Datta, K., Shah, P., Srivastava, T., Mathur, S. G., Chattopadhyay, P. and Sinha, S. Sensitizing glioma cells to cisplatin by abrogating the p53 response with antisense oligonucleotides. Cancer Gene Therapy. 2004. 11, 525-531. [PubMed]
29.
Srivastava, T., Chattopadhyay, P., Mahapatra, A. K., Sarkar, C. and Sinha, S. Increased hMSH2 protein expression in glioblastoma multiforme. Journal of Neuro-oncology. 2004. 66, 51-57. [PubMed]
30.
Bose, B., Chugh, D. A., Kala, M., Acharya, S. K., Khanna, N. and Sinha, S. Characterization and molecular modeling of a highly stable anti-Hepatitis B surface antigen scFv. Molecular Immunology. 2003. 40, 617-631. [PubMed]
31.
Datta, K., Mathur, S. G., Srivastava, T., Shah, P., Chattopadhyay, P. and Sinha, S. Hydroxylamine potentiates the effect of low dose hydrogen peroxide in glioma cells independent of p53. The International Journal of Biochemistry & Cell Biology. 2003. 35, 1639-1644. [PubMed]
32.
Sarkar, C., Chattopadhyay, P., Ralte, A. M., Mahapatra, A. K. and Sinha, S. Loss of heterozygosity of a locus in the chromosomal region 17p13.3 is associated with increased cell proliferation in astrocytic tumors. Cancer Genetics and Cytogenetics. 2003. 144, 156-164. [PubMed]
33.
Kala, M., Misra, A., Saini, D., Bajaj, K. and Sinha, S. Phage displayed antibodies to heat stable alkaline phosphatase: framework region as a determinant of specificity. Journal of Biochemistry. 2002. 132, 535-541. [PubMed]
34.
Datta, K., Babbar, P., Srivastava, T., Sinha, S. and Chattopadhyay, P. p53 dependent apoptosis in glioma cell lines in response to hydrogen peroxide induced oxidative stress. The International Journal of Biochemistry & Cell Biology. 2002. 34, 148-157. [PubMed]
35.
Misra, A., Chosdol, K., Sarkar, C., Mahapatra, A. K. and Sinha, S. Alteration of a sequence with homology to human endogenous retrovirus (HERV-K) in primary human glioma: implications for viral repeat mediated rearrangement. Mutation Research. 2001. 484, 53-59. [PubMed]
36.
Jotwani, G., Misra, A., Chattopadhyay, P., Sarkar, C., Mahapatra, A. K. and Sinha, S. Genetic heterogeneity and alterations in chromosome 9 loci in a localized region of a functional pituitary adenoma. Cancer Genetics and Cytogenetics. 2001. 125, 41-45. [PubMed]
37.
Kala, M., Bajaj, K. and Sinha, S. Direct antigen capture by soluble scFv antibodies. A method for detection, characterization, and determination of affinity. Applied Biochemistry and Biotechnology. 2001. 90, 11-22. [PubMed]
38.
Misra, A., Chattopadhyay, P., Dinda, A. K., Sarkar, C., Mahapatra, A. K., Hasnain, S. E. and Sinha, S. Extensive intra-tumor heterogeneity in primary human glial tumors as a result of locus non-specific genomic alterations. Journal of Neuro-oncology. 2000. 48, 1-12. [PubMed]
39.
Datta, K., Sinha, S. and Chattopadhyay, P. Reactive oxygen species in health and disease. The National Medical Journal of India. 2000. 13, 304-310. [PubMed]
40.
Das, N. and Sinha, S. Problem-oriented small-group discussion in the teaching of biochemistry laboratory practicals. Biochemical Education. 2000. 28, 154-155. [PubMed]
41.
Kala, M., Bajaj, K. and Sinha, S. Magnetic bead enzyme-linked immunosorbent assay (ELISA) detects antigen-specific binding by phage-displayed scFv antibodies that are not detected with conventional ELISA. Analytical Biochemistry. 1997. 254, 263-266. [PubMed]
42.
Chattopadhyay, P., Rathore, A., Mathur, M., Sarkar, C., Mahapatra, A. K. and Sinha, S. Loss of heterozygosity of a locus on 17p13.3, independent of p53, is associated with higher grades of astrocytic tumours. Oncogene. 1997. 15, 871-874. [PubMed]
43.
Das, N. and Sinha, S. Problem based module for the learning of biochemistry and molecular biology of hepatitis. Tropical Gastroenterology. 1997. 18, 87-90. [PubMed]
44.
Joshi, A. R., Sinha, S. , Sulaiman, I. M., Banerji, A. K. and Hasnain, S. E. Alterations in brain tumor DNA detected by a fingerprinting probe. Indian Journal of Biochemistry & Biophysics. 1996. 33, 455-457. [PubMed]
45.
Sinha S. Malaria and human evolution. The National Medical Journal of India. 1996. 9, 176-177. [PubMed]
46.
Chattopadhyay, P., Banerjee, M., Sarkar, C., Mathur, M., Mohapatra, A. K. and Sinha, S. Infrequent alteration of the c-myc gene in human glial tumours associated with increased numbers of c-myc positive cells. Oncogene. 1995. 11, 2711-2714. [PubMed]
47.
Kumar, A., Kumar, A., Sinha, S. , Khandekar, P. S., Banerjee, K. and Srivastava, L. M. Hind III genomic polymorphism of the C3b receptor (CR1) in patients with SLE: low erythrocyte CR1 expression is an acquired phenomenon. Immunology and Cell Biology. 1995. 73, 457-462. [PubMed]
48.
Sinha S. Genetics for clinicians. Tropical Gastroenterology. 1995. 16, 138-140. [PubMed]
49.
Chattopadhyay, P. and Sinha, S. Adaptation of a chemiluminescent DNA detection system to strongly positively charged nylon membranes: single copy gene detection in human genome. Indian Journal of Biochemistry & Biophysics. 1995. 32, 152-155. [PubMed]
50.
Sinha S. TNF-alpha alleles and susceptibility to cerebral malaria. The National Medical Journal of India. 1995. 8, 70-72. [PubMed]
51.
Ghosh, M., Dinda, A., Chattopadhyay, P., Sarkar, C., Bhatia, S. and Sinha, S. Rearranged p53 gene with loss of normal allele in a low-grade nonrecurrent glioma. Cancer Genetics and Cytogenetics. 1994. 78, 68-71. [PubMed]
52.
Kumar, A., Malaviya, A. N., Sinha, S., Khandekar, P. S., Banerjee, K. and Srivastava, L. M. C3b receptor (CR1) genomic polymorphism in rheumatoid arthritis. Low receptor levels on erythrocytes are an acquired phenomenon. Immunologic Research. 1994. 13, 61-71. [PubMed
Book Chapters:
Sinha, S. , Ramachandran, L.K., Proteins: Chemistry-Structure-Conformation. In: Textbook of Biochemistry and Human Biology, Talwar, G.P. and Srivastava, L.M. Eds., Prentice-Hall of India Pvt Ltd, New Delhi. 2003. pp 47-61.
Grant Support:
Project Title Role Funding Agency Sanction Number Sanction Date
An in vitro study of the role of FAT, a Drosophilla tumour suppressor gene homologue in human glial tumorigenesis
Defence Research and Development Organisation, New Delhi, India
Generation and characterization of phage displayed recombinant antibodies to biochemically and immunologically relevant epitopes of the oncodevelopmental antigen heat stable alkaline phosphatase
Indian Council of Medical Research
2006-2009
Ligand-based selection from combinatorial libraries for generating recombinant antibodies to the placental (oncofoetal) isozyme of alkaline phosphatase: Characterization and validation in clinical cancers
Department of Biotechnology, Government of India
2005-2008
Expression of recombinant antibody in E. coli: Processes for improving binding characteristics of antibodies to hepatitis B surface antigen (HbsAg)
Department of Biotechnology, Government of India
2002-2006
Interaction of DNA repair genes and p53 in determining the cellular response to genotoxic agents in glial tumour cell-lines
Department of Science & Technology, Government of India
Deregulated initiation of translation in oral carcinogenesis
Department of Science & Technology, Government of India
Education
::
M.D.
::
Ph.D.
Honors and Awards
::
Fellow, National Academy of Medical Sciences
::
Fellow, Indian National Science Academy
Member
::
Scientific Advisory Committee of the Institute of Cytology and Preventive Oncology, Delhi
::
2006-07, Academic Committee, Centre for Cellular and Molecular Biology, Jawaharlal Nehru University
::
2005–07, Project Review Committee, Chronic Diseases, Department of Biotechnology
::
2006, Co-convener, NII–AIIMS symposium on Translational Research, New Delhi
::
2005-till date, Project Review Committee, Biotechnology Products and Process Development, Department of Biotechnology
::
2005, Project Review Committee, Cell and Molecular Biology, Indian Council of Medical Research
::
2003–05, Academic Council, Jawaharlal Nehru University
::
2001-till date, Institutional Ethics Committee, Jawaharlal Nehru University
::
2001, Special Technical Committee, Task Force on Biotechnology Process and Product Development, Department of Biotechnology
::
2001-till date, Special Committee, Centre for Molecular Medicine, Jawaharlal Nehru University
::
2001, Special Committee, Centre for Molecular Medicine and Centre for Biotechnology
::
2000 till date, Special Committee, Centre for Biotechnology, Jawaharlal Nehru University
::
2000, Institutional Biosafety Committee and Ethical Committee of the National Institute of Immunology
::
2000-till date, Institutional Biosafety Committee and Ethical Committee of the National Institute of Immunology
::
2000, Project Review Committee, for Cancer and Cell and Molecular Biology, Indian Council of Medical Research
::
Research Council, SGPGIMS, Lucknow Chairman
::
Scientific Advisory Committee, Rajiv Gandhi Centre of Biotechnology, Thiruvananthapuram
::
Scientific Advisory Committee, Institute of Pathology, New Delhi
::
Scientific Advisory Committee, School of Medical Sciences, University of Hyderabad
::
Scientific Advisory Committee of the Institute of Cytology and Preventive Oncology, Delhi
Type of Neoplasms
Head and Neck
Central nervous system
Soft tissues tumor
Nerve tissue